GC-MS Analysis of Suaeda maritima and its Application as a Salt Substitute in Fish Marinade Powder
Keywords:
Bioactive compounds, Fish, GC-MS, Marinate powder, SeabliteAbstract
This study aims to identify bioactive compounds in seablite (Suaeda maritima) and develop seablite-based fish powder seasoning and marinade as an alternative low-sodium product. The chemical composition of edible seablite extract was analyzed using gas chromatography-mass spectrometry (GC-MS). Production process: powder preparation from dried seablite leaves and dried herbal powder including 5% lemongrass, 5% galangal, 5% garlic, and 5% kaffir lime leaves. Sensory evaluation and consumer acceptance test were conducted on 30 participants. GC-MS results showed that seablite extract contains phytochemicals, with the main components including phytol (43.19%), Myo-Inositol (7.06%), 9,12,15-Octadecatrienoic acid, (Z,Z,Z)- (6.32%), 9-Octadecenamide, (Z)- (5.36%) and Palmitoleamide (5.21%). The final product contained 320.87 kcal, 8.65 g protein, 68.62 g carbohydrate, 1.31 g fat, 15.19 g ash, 6.23 g water per 100 grams, and 46,431.25 mg/kg sodium. This formulation offers a healthier alternative with reduced sodium while maintaining flavor and nutritional value. The optimal formulation for seasoning and marinating fish powder consisted of 50% dried seablite powder. Sensory evaluation scores indicated consumer preferences ranging from “somewhat liked” to “quite liked.” These findings indicate that the developed seasoning powder is a promising, tasty, and healthier alternative for low-sodium fish products, while maintaining acceptable sensory qualities.
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References
Wang, L., Du, J., Cao, W., and Sun, S. (2021). Trends of stroke attributable to high sodium intake at the global, regional, and national levels from 1990 to 2019: a population-based study. Neurological Research, 43(6), 474–481.
Yin, X., Tian, M., and Neal, B. (2021). Sodium reduction: how big might the risks and benefits be?. Heart, Lung and Circulation, 30(2), 180-185.
World Health Organization. (2026, March 15). Guideline: sodium intake for adults and children. https://www.who.int/publications/i/item/9789241504836.
Chokumnoyporn, N., Sriwattana, S., Phimolsiripol, Y., Torrico, D.D., and Prinyawiwatkul, W. (2015). Soy sauce odour induces and enhances saltiness perception. International Journal of Food Science and Technology, 50(10), 2215-2221.
Nurmilah, S., Cahyana, Y., Utama, G. L., and Aït-Kaddour, A. (2022). Strategies to reduce salt content and its effect on food characteristics and acceptance: a review. Foods, 11(19), 3120.
Wilailux, C., Sriwattana, S., Chokumnoyporn, N., and Prinyawiwatkul, W. (2019). Texture and colour characteristics, and optimisation of sodium chloride, potassium chloride and glycine of reduced-sodium frankfurter. International Journal of Food Science and Technology, 55(5), 2232-2241.
Taladrid, D., Laguna, L., Bartolomé, B., and Moreno-Arribas, M. (2020). Plant-derived seasonings as sodium salt replacers in food. Trends in Food Science and Technology, 99, 194-202.
Singti, W., Prachongpun, P., Sarasuk, C., Ngoenchai, P., and Niamsuk, J. (2026). Sodium-reduced bread formulated with seablite (Suaeda maritima L. Dumort) powder as salt substitution. Brazilian Journal of Food Technology, 29, e2025047.
Sangray, R., Thongngamdee, S., and Inyoo, A. (2015). Study of seablite to construct science laboratory direction for Prathomsuksa 6 students. Journal for Social Sciences Research, 6(1), 101-120.
Matra, S., and Charenrat, J. (2014). Oral rehydration salts of suaeda maritima. (Research Report). Rajamangala University of Technology Phra Nakhon, Bangkok.
Pornpitakdamrong A., and Sudjaroen, Y. (2014). Seablite (Suaeda maritima) product for cooking, Samut songkram province, Thailand. Food and Nutrition Sciences, 5(9), 850-856.
Sudjaroen, Y. (2015). Evaluation for nutritive values and antioxidant activities of dried Seablite (Suaeda maritima). Scientific Research and Essays, 10(9), 306-312.
Thongkao, K., Kakatum, N., and Sudjaroen, Y. (2022). Antioxidant and anti-inflammation activities of ethanol extract from Seablite (Suaeda Maritima) root. Journal of Pharmaceutical Negative Results, 13(3), 101-104.
Dzhoglova, V., Ivanov, K., Karcheva - Bahchevanska, D., Koleva, N., Bozhkova, M., Benbassat, N., and Ivanova, S. (2024). Salicornia europaea L. and Suaeda maritima (L.) Dumort: bioactive compounds and future perspectives. Biotechnology & Biotechnological Equipment, 38(1), 2326291.
Manojkumar, S., Thandeeswaran, M., Thangavel, S.K., Arjunan, A., Muthuselvam, M., Kalaiarasi, G. and Gnanajothi, K. (2024). Phytochemical screening, in silico molecular docking, ADME properties, and in vitro antioxidant, anticancer, and antidiabetic activity of marine halophyte Suaeda maritima (L.) Dumort. ACS omega, 9(10), 11200-11216.
Islam, M.N., and Molinar-Toribio, E.M. (2013). Development of a meat tenderizer based on papaya peel. Revista I+D Tecnológico, 9(2), 24-29.
Saranya, S., Santhi, D., and Kalaikannan, A. (2016). Ginger as a tenderizing agent for tough meats -A review. Journal of Livestock Science, 7, 54-61.
Clavel-Coibrié, E., Sales, J. R., da Silva, A. M., Barroca, M. J., Sousa, I., and Raymundo, A. (2021). Sarcocornia perennis: A salt substitute in savory snacks. Foods, 10(12), 3110.
Srivarathan, S., Olarte Mantilla, S. M., Phan, A. D. T., Wright, O. R., Sultanbawa, Y., and Netzel, M. E. (2024). Assessing the potential of Australian indigenous edible halophytes as salt substitutes: From wild to plate. Journal of Food Science, 89(5), 2991-3005.
Tongkam, S., Chokumnoyporn, N., and Chum-in, T. (2022). Development approach of marinade powder from suaeda maritima for fish. Journal of Applied Research on Science and Technology, 21(1), 74-87.
Ministry of Public Health. (2025, July 15). The Ministry of Public Health Announcement No. 182. Retrieved from http://www.ratchakitcha.soc.go.th/DATA/PDF/2541/D/047/ 23.PDF.
Peryam, D.R., and Pilgrim, P.J. (1957). Hedonic scale method of measuring food preference. Food Technology, 11, 9-14.
Deesawat, U. (2007). Tom Yum Kung Chili Paste. (Research Report). Rajamangala University of Technology Krungthep, Bangkok.
Somalee, C., and Pitchairat, D. (2017). Product Development of ready to eat tom-yum seasoned hard clam (Meretrix casta) with herbal supplement for fishing community in Trang province. RMUTSV Research Journal, 9(2), 307-321.
Boonbumrung, S. (2006). Study on aroma functional properties of Thai Tom Yum essential oils. (Research Report). Kasetsart University, Bangkok.
AOAC. (2019). Official Method of Analysis of AOAC International. USA., AOAC International.
Chhetri, D.R. (2019). Myo-inositol and its derivatives: their emerging role in the treatment of human diseases. Frontiers in Pharmacology, 10, 1172.
Marandi, R.R., Britto, S.J., and Soreng, P.K. (2018). FT-IR, HPLC, GC-MS and wis of Peucedanum dhana buch.-Ham. Ex CB Clarke (Bhojraj): A rare and endangered medicinal plant of Chotanagpur, Jharkhand. International Journal of Research in Pharmacy and Pharmaceutical Sciences, 3(1), 119-126.
De Castro Oliveira, L.G., Brito, L.M., De Moraes Alves, M.M., Amorim, L.V., Sobrinho-Júnior, E.P.C., De Carvalho, C.E.S., Da Franca Rodrigues, K.A., Arcanjo, D.D.R., Das Graças Lopes Citó, A.M., and De Amorim Carvalho, F.A. (2017). In vitro effects of the neolignan 2, 3-dihydrobenzofuran against Leishmania amazonensis. Basic & Clinical Pharmacology & Toxicology, 120(1), 52–58.
Hongal, A.M., Shettar, A.K., Hoskeri, J.H., and Vedamurthy, A.B. (2024). GCMS-based phytochemical profiling and in vitro pharmacological activities of plant Alangium salviifolium (L.f) Wang. Future Journal of Pharmaceutical Sciences, 10, 61.
Lapczynski, A., Bhatia, S.P., Letizia, C.S., and Api, A.M. (2008). Fragrance material review on 3, 7-dimethyloct-7-en-1-ol. Food and chemical toxicology, 46(11), S144-S146.
Api, A.M., Bartlett, A., Belsito, D., Botelho, D., Bruze, M., Bryant-Friedrich, A., Burton, G.A. Jr, Cancellieri, M.A., Chon, H., Dagli, M.L., Dekant, W., Deodhar, C., Farrell, K., Fryer, A.D., Jones, L., Joshi, K., Lapczynski, A., Lavelle M, Lee, I., Moustakas, H., Muldoon, J., Penning, T.M., Ritacco, G., Sadekar N, Schember, I, Schultz, T.W., Siddiqi, F., Sipes, I.G., Sullivan, G., Thakkar, Y., and Tokura, Y. (2025). RIFM fragrance ingredient safety assessment, tridecanal, CAS registry number 10486-19-8. Food and Chemical Toxicology, 197, 115180.
Nabavi, S.F., Maggi, F., Daglia, M., Habtemariam, S., Rastrelli, L., and Nabavi, S.M. (2016). Pharmacological Effects of Capparis spinosa L. Phytotherapy Research, 30(11), 1733-1744.
Dias, M.K.H.M., Madusanka, D.M.D., Han, E.J., Kim, M.J., Jeon, Y.J., Kim, H.S., Fernando, I.P.S., and Ahn, G. (2020). (-)-Loliolide isolated from sargassum horneri protects against fine dust-induced oxidative stress in human keratinocytes. Antioxidants, 9(6), 474.
Grabarczyk, M., Wińska, K., Mączka, W., Potaniec, B. and Anioł, M. (2015). Loliolide-the most ubiquitous lactone. Acta Universitatis Lodziensis. Folia Biologica Et Oecologica, 11, 1-8.
Li, L.L., Zhao, H.H., and Kong, C.H. (2020). (–)-Loliolide, the most ubiquitous lactone, is involved in barnyardgrass-induced rice allelopathy. Journal of Experimental Botany, 71(4), 1540-1550.
Ganesan, T., Subban, M., Christopher Leslee, D.B., Kuppannan, S.B., and Seedevi, P. (2024). Structural characterization of n-hexadecanoic acid from the leaves of Ipomoea eriocarpa and its antioxidant and antibacterial activities. Biomass Conversion and Biorefinery, 14(13), 14547-14558.
Purushothaman, R., Vishnuram, G., and Ramanathan, T. (2025). Antiinflammatory efficacy of n-Hexadecanoic acid from a mangrove plant Excoecaria agallocha L. through in silico, in vitro and in vivo. Pharmacological Research-Natural Products, 7, 100203.
Santos, C.C.D.M.P., Salvadori, M.S., Mota, V.G., Costa, L.M., De Almeida, A.A.C., De Oliveira, G.A.L., Costa, J.P., De Sousa, D.P., De Freitas, R.M., and De Almeida, R.N. (2013). Antinociceptive and antioxidant activities of phytol in vivo and in vitro models. Neuroscience journal, 2013(1), 949452.
Al-Garawi, N.I., Abu-Serag, N.A., Alee Shaheed, K.A., and Bahadly, Z.K.A. (2019). Analysis of bioactive phytochemical compound of (Cyperus alternifolius L.) by using gas chromatography–mass spectrometry. IOP conference series: Materials science and engineering, IOP Publishing, 012047.
Sahoo, C.R., Paidesetty, S.K., and Padhy, R.N. (2021). The recent development of thymol derivative as a promising pharmacological scaffold. Drug Development Research, 82(8), 1079-1095.
Venceslau, A.D.F.A., Mendonça, A.C., Benedick, L.A.Z., Thomasi, S.S., Nunes, C.A., and Pinto, L.M.A. (2021). Analysis of the chemical constituents of Thompson atemoya seed oil. Revista Brasileira de Fruticultura, 43(6), e-015.
Akanmu, M.A., Adeosun, S.O., and Ilesanmi, O.R. (2007). Neuropharmacological effects of oleamide in male and female mice. Behavioural Brain Research, 182(1), 88-94.
Driscoll, W.J., Chaturvedi, S., and Mueller, G.P. (2007). Oleamide synthesizing activity from rat kidney: identification as cytochrome c. Journal of Biological Chemistry, 282(31), 22353-22363.
Tanvir, R., Javeed, A., and Rehman, Y. (2018). Fatty acids and their amide derivatives from endophytes: New therapeutic possibilities from a hidden source. FEMS Microbiology letters, 365(12), fny114.
Ganiyat, K. O., Patricia, A. O., and Sunday, F. A. (2011). Chemical composition, toxicity, antimicrobial and antioxidant activities of leaf and stem essential oils of Dieffenbachia picta (Araceae). European Journal of Scientific Research, 49(4), 567–580.
Al-Shawi, A.A., Hameed, H.F., Ali, N.H., and Hussein, K.A. (2020). Investigations of phytoconstituents, antioxidant and anti-liver cancer activities of Saueda monoica Forssk extracted by microwave-assisted extraction. Asian Pacific Journal of Cancer Prevention: APJCP, 21(8), 2349.
Amin, E., Elwekeel, A., Alshariedh, N.F., Abdel-Bakky, M.S., and Hassan, M.H. (2022). GC-MS Analysis and Bioactivities of the Essential Oil of Suaeda aegyptiaca. Separations, 9(12), 439.
Kim, D.H., Mahomoodally, M.F., Sadeer, N.B., Seok, P.G., Zengin, G., Palaniveloo, K., Khalil, A.A., Rauf, A., and Rengasamy, K.R. (2021). Nutritional and bioactive potential of seagrasses: A review. South African Journal of Botany, 137, 216-227.
Matloub, A.A., Hamed, M.A., and El Soud, S.S. (2014). Chemo-protective effect on hepato-renal toxcicity and cytotoxic activity of lipoidal matter of Atriplex lindleyi Moq. International Journal of Pharmacy and Pharmaceutical Sciences, 6(8), 187-196.
Rodrigues, M.J., Jekő, J., Cziáky, Z., Pereira, C.G., and Custódio, L. (2022). The medicinal halophyte Frankenia laevis L.(sea heath) has in vitro antioxidant activity, α-glucosidase inhibition, and cytotoxicity towards hepatocarcinoma cells. Plants, 11(10), 1353.
Nayak, B., Roy, S., Roy, M., Mitra, A., and Karak. K. (2018). Phytochemical, antioxidant and antimicrobial screening of Suaeda maritima L (Dumort) against human pathogens and multiple drug resistant bacteria. The Indian Journal of Pharmaceutical Sciences, 80(1), 26-35.
Wang, N., Tello, E., and Peterson, D. G. (2025). Identification of a salt-enhancing compound in thyme using sensory-guided preparative-LC fractionation. Food Research International, 221(3), 117467.
Rosa, A., Pinna, I., Piras, A., Porcedda, S., and Masala, C. (2022). Flavoring of sea salt with Mediterranean aromatic plants affects salty taste perception. Journal of the Science of Food and Agriculture, 102(13), 6005-6013.
Shahidi, F., and Hossain, A. (2022). Role of lipids in food flavor generation. Molecules, 27(15), 5014.
Spence, C. (2015). Multisensory flavor perception. Cell, 161(1), 24–35.
Sood, S., Methven, L., Balagiannis, D. P., and Cheng, Q. (2024). Can samphire be the new salt? Understanding the potential of samphire harvested from the UK coastline. Food Chemistry, 438, 138065.
Ventura, Y., Wuddineh, W.A., Myrzabayeva, M., Alikulov, Z., Khozin-Goldberg, I., Shpigel, M., Samocha, T.M., and Sagi, M. (2011). Effect of seawater concentration on the productivity and nutritional value of annual Salicornia and perennial Sarcocornia halophytes as leafy vegetable crops. Scientia Horticulturae, 128(3), 189-196.
Barreira, L., Resek, E., Rodrigues, M.J., Rocha, M.I., Pereira, H., Bandarra, N., da Silva, M.M., Varela, J. and Custódio, L. (2017). Halophytes: Gourmet food with nutritional health benefits. Journal of Food Composition and Analysis, 59, 35–42.
Anderson, C.A., Cobb, L.K., Miller III, E.R., Woodward, M., Hottenstein, A., Chang, A.R., Mongraw-Chaffin, M., White, K., Charleston, J., Tanaka, T., Thomas, L., and Appel, L. J. (2015). Effects of a behavioral intervention that emphasizes spices and herbs on adherence to recommended sodium intake: results of the SPICE randomized clinical trial. The American Journal of Clinical Nutrition, 102(3), 671-679.
Tomić-Obrdalj, H., Keser, I., Ranilović, J., Palfi, M., Gajari, D., and Cvetković, T. (2023). The use of herbs and spices in sodium-reduced meals enhances saltiness and is highly accepted by the elderly. Food Quality and Preference, 105, 104789.
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